Release of dipicolinic acid from spores of Geobacillus stearothermophilus

Induced release of dipicolinic acid from spores of Bacillus megaterium.

Conditions under which dipicolinic acid may be released from bacterial spores probably relate to the chemical state of the dipicolinic acid in the spore, to its physical location in the spore, and to its influence on the physiological properties of the spore. The latter aspect embraces the relation between release of dipicolinic acid and the viability of the spore, its heat resistance, and its ...

Monoethyl ester of dipicolinic acid from bacterial spores.

The discovery and certain aspects of the biosynthesis of dipicolinic acid (pyridine-2,6-dicarboxylic acid) in exudates of germinated spores and in intact spores of aerobic bacilli have been recently reported (Powell, 1953; Perry and Foster, 1955). In the latter paper it was noted that maximum recovery of dipicolinic acid from intact spores necessitated an initial treatment with hot dilute inorg...

Liquid chromatographic determination of dipicolinic Acid from bacterial spores.

Dipicolinic acid was determined by reverse-phase liquid chromatography. Elution was with 0.2 M potassium phosphate, pH 1.8, containing 1.5% tert-amyl alcohol or higher concentrations of lower alcohols or acetonitrile. The normal analytical range was 50 to 1,000 muM, which is equivalent to 0.1 to 1 mg of spores per ml with a relative standard error of 2 to 4% and a detection limit of <100 pmol. ...

Relation of dipicolinic acid to heat resistance of bacterial spores.

Dipicolinic Acid Release and the Germination of Alicyclobacillus acidoterrestris Spores under Nutrient Germinants.

The presence of Alicyclobacillus, a thermoacidophilic and spore-forming bacterium, in acidic fruit juices poses a serious problem for the processing industry. A typical sign of spoilage in contaminated juices is a characteristic phenolic off-flavour associated with the production of guaiacol. Spores are formed in response to starvation and in a natural environment re-access the nutrients, e.g.:...